PCR amplification was performed in a total volume of 10 μl, containing 1 μl cDNA, 1 μl 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U ExTaq polymerase (Takara Bio, Inc.), 500 nM each ...

2025年8月10日，北京大学/昌平实验室魏文胜团队在Nature Communications发表研究论文，基于一型与二型内含子的第二步剪接反应 ...

Within multi-exon genes, after introns are removed, the exons must be spliced back together. Splice-enhancer domains (exon sequences near the intron–exon boundary) help to ensure that this happens ...

During RNA maturation, the group I intron promotes two sequential phosphorotransfer reactions resulting in exon ligation and intron release. Here, we report the crystal structure of the intron in ...

This is a preview. Log in through your library . Abstract RNA is commonly believed to undergo a number of sequential folding steps before reaching its functional fold, i.e., the global minimum in the ...

Researchers at University of Tsukuba have decoded the nuclear genome of Amorphochlora amoebiformis, a unicellular marine alga belonging to the chlorarachniophyte group. The work is published in the ...