Major hurdles in early-stage drug discovery include how to triage hit compounds identified from cell-based screens, as well as how to rapidly evaluate their cellular efficacy and pinpoint side effects ...

We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART ™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA by 454 ...